Heat inactivation partially preserved barrier and immunomodulatory effects of <i>Lactobacillus gasseri</i> LA806 in an <i>in vitro</i> model of bovine mastitis

Probiotics could help combat infections and reduce antibiotic use. As use of live bacteria is limited in some cases by safety or regulatory concerns, the potential inactivated worth investigating. We evaluated heat-inactivated Lactobacillus gasseri LA806 to counteract Staphylococcus aureus Escherichia coli infection cycles an vitro model bovine mastitis. assessed ability impair pathogen colonisation mammary epithelial cells (bMECs) modulate cytokine expression pathogen-stimulated bMECs. Live induced a five-fold decrease S. adhesion internalisation (while not affecting E. colonisation) decreased pro-inflammatory aureus-stimulated bMECs (without interfering with immune response coli). The diminish was two-fold lower than that strain, but its anti-inflammatory properties were barely impacted. Even though effects impaired after inactivation, both have barrier immunomodulatory be useful gland. involved various types infection, would exploring other contexts.